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KMID : 0545120070170122018
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Journal of Microbiology and Biotechnology
2007 Volume.17 No. 12 p.2018 ~ p.2026
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Swinging Effect of Salicylic Acid on the Accumulation of Polyhydroxyalkanoic Acid (PHA) in Pseudomonas aeruginosa BM114 Synthesizing Both MCLandSCL-PHA
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Rho Jong-Kook
Choi Mun-Hwan
Shim Ji-Hoon
Lee So-Young
Ko Bong-Sung
Chi Ki-Whan
Yoon Sung-Chul
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Abstract
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A bacterium, Pseudomonas aeruginosa BM114, capable of accumulating a blend of medium-chain-length (MCL)- and short-chain-length (SCL)-polyhydroxyalkanoic acid (PHA), was isolated. Salicylic acid (SA), without being metabolized, was found to specifically inhibit only the accumulation of MCL-PHA without affecting cell growth. An addition of 20 mM SA selectively inhibited the accumulation of MCL-PHA in decanoate-grown cells by 83% of the control content in one-step cultivation, where overall PHA accumulation was inhibited by only ~11%. Typically, the molar monomerunit ratio of the PHA for 25 mM decanoate-grown cells changed from 46:4:25:25 (=[3-hydroxybutyrate]:[3-hydroxycaproate]: [3-hydroxyoctanoate]:[3-hydroxydecanoate]) at 0 mM SA (dry cell wt, 1.97 g/l; PHA content, 48.6 wt%) to 91:1:4:4 at 20 mM SA (dry cell wt, 1.85 g/l; PHA content, 43.2 wt%). Thus, the stimulation of SCL-PHA accumulation was observed. Growth of P. aeruginosa BM114 on undecanoic acid also produced a PHA blend composed of 47.4% P(3HB-co-3- hydroxyvalerate) and 52.6% P(3-hydroxyheptanoate-co-3- hydroxynonanoate-co-3-hydroxyundecanoate). Similar to the case of even-carboxylic acids, SA inhibited the accumulation of only MCL-PHA, but stimulated the accumulation of SCLPHA. For all medium-chain fatty acids tested, SA induced a stimulation of SCL-PHA accumulation in the BM114 strain. SA could thus be used to suppress only the formation of MCL-PHA in Pseudomonas spp. accumulating a blend of SCL-PHA and MCL-PHA.
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KEYWORD
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Polyhydroxyalkanoic acid, PHA accumulation inhibitor, salicylic acid, SCL-PHA accumulation stimulator
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